花生ISSR分子标记技术初步研究

Preliminary Identification of Peanut Varieties by ISSR Molecular Markers

以花生为研究对象,初步建立起花生的ISSR分子标记技术体系,包括DNA提取、DNA模板浓度、引物浓度、退火温度、循环次数和引物筛选等。结果表明:从48条引物中选择4个条带清晰的引物对7个品种进行扩增,共检测出15条带,其中多态性带为12条,多态性比例为80%,能把7个花生品种区分开来。本试验为花生品种鉴定和新品种选育提供理论依据。

The study aimed to set up the ISSR reaction system of peanut, include the genomic DNA extraction, DNA template concentration, primer concentration, annealing temperatare, cycle time, primers screening. The results showed that 4 primers were screened out from 48 ISSR primers totally to amplify the peanut genome DNAs of 7 materials effectively and 15 sites were amplified out totally , including 12 polymorphism sites with polymorphism rate of 80%. All of the 7 varieties could be differentiated from each other based on the fingerprints established by these specific bands. The results provided a theoretical to seed purity identification and new varieties breeding of peanut.