摘要
【目的】以小白鼠为实验动物模型、玉米秸秆为发酵床垫料,探讨发酵床垫料中的微生物对小鼠机体免疫功能的影响。【方法】将小鼠随机分为4组,第1—3组小鼠分别置于用不同微生物配方发酵的玉米秸秆垫料中饲养,第4组作为对照组,放入未发酵的垫料中饲喂。期间检测小鼠血清中的抗体效价、IL-4和IFN-γ的含量,血液中白细胞总数、淋巴细胞数量、红细胞总数及小鼠盲肠内乳酸菌数量,抗疲劳游泳时间等指标。【结果】发酵垫料组微生物能显著提高小鼠抗体效价和血液中红细胞数量、血红蛋白浓度及盲肠乳酸菌数量(P<0.05);可增加血清中IFN-γ、IL-4含量和血液中白细胞总数、淋巴细胞数量,差异不显著(P>0.05);抗疲劳试验结果表明,发酵垫料微生物能显著延长小鼠的游泳时间(P<0.05)。【结论】玉米秸秆发酵垫料微生物可显著提高动物体的免疫功能及健康状况。
[ Objective ] The aim of this experiment was to study the effect of microbes in fermented corn stalk on immunologic function of mice.[Method] Forty mice at 4-week-old of age were divided into four groups randomly and raised to establish different kinds of fermention corn stalk with microbes group as well as control group (without addition of microbes). In the experiments, the antibody titer, contents of IL-4 and IFN-γ in serum, the total number of white blood cell (WBC), lymphocyte (LYM) and red blood cell (RBC), and mean corpuscular haemoglobin concentration (MCHC) in blood were determined. The count of Lactobacillus in cecal content and the anti-fatigue time of swimming of mice were examined. [ Result ] The results showed that microbes in fermented corn stalk significantly promoted the level of antibody and the count of Lactobacillus in cecal content, RBC and MCHC in blood (P 〈0.05). The WBC and LYM in blood, the content of IFN-γ, IL-4 were also increased (P〉0.05). The result of anti-fatigue experiment showed that the microbes in fermented corn stalk significantly increased the time of swimming of mice (P〈0.05). [Conclusion] Microbes in fermented corn stalk can significantly improve the immune function of mice and other indicators.
出处
《中国农业科学》
CAS
CSCD
北大核心
2011年第16期3463-3468,共6页
Scientia Agricultura Sinica
基金
国家"十一五"科技支撑计划项目(2006BAD06A11)
关键词
发酵玉米秸秆
微生物
小鼠
免疫功能
抗体
细胞因子
fermention corn stalk
microbes
mice
immunologic function
antibody
cytokines