摘要
目的观察隐丹参酮(CT)对谷氨酸(Glu)损伤大鼠皮层神经元的保护作用。方法体外培养大鼠皮层神经细胞,建立Glu损伤模型,采用MTT法检测细胞活力,比色法检测超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;荧光法检测细胞内Ca2+的变化,Western blot检测caspase 3蛋白的表达。结果 CT能显著提高Glu损伤神经细胞的存活率,提高SOD的活性,降低MDA的生成,抑制细胞内[Ca2+]i的变化以及caspase 3蛋白表达,抑制神经细胞凋亡。结论 CT对培养神经细胞谷氨酸兴奋毒性具有显著的保护作用,其机制可能与CT拮抗Glu诱导的氧化应激以及抑制细胞内[Ca2+]i作用有关。
OBJECTIVE To investigate the protective effect of cryptotanshinone on injury induced by glutamatic acid in rats. METHODS Cell viability was detected by MTT assay. Superoxide dismutase （SOD）activity and malondialdehvde （MDA）level were detected by colorimetry, The concentration of intracellular Ca^2＋ was detected with fluorescence probe. And the expression of caspase 3 was detected by Western blot. RESULTS Compared with glutamaic acid group, cryptotanshinone could increase cell survival rate and the SOD activity, decrease the level of MDA and cell apoptosis as well as caspase 3 expression,and decrease intracellular Ca^2＋ accu- mulation. CONCLUSION Cryptotanshinone could protect rat cortical neurons from Glu-induced neurotoxicity. This protection is possibly related with suppression of the generation of lipid peroxide and inhibition of intracellular Ca^2＋ accumulation.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2011年第16期1245-1248,共4页
Chinese Pharmaceutical Journal
基金
国家自然科学基金(30772576)
科技部重大新药创制临床前研究专项(2009ZX09102-152)
关键词
隐丹参酮
谷氨酸
皮层神经元
凋亡
cryptotanshinone
glutamate
cortical neurons
apoptosis
