HPLC-PAD测定刺五加中刺五加苷B与刺五加苷E的含量

Determination of Eleutheroside B and Eleutheroside E in Acanthopanax senticosus by HPLC-PAD

目的研究HPLC同时测定刺五加中刺五加苷B与刺五加苷E的含量。方法采用Agilent Zorbax SB-C18柱(4.6mm×250 mm,5μm),流动相为乙腈-0.5%磷酸(0～10 min,9∶91;10～30 min,9～20∶91～80),流速1.0 mL.min-1,检测波长220 nm,柱温25℃。结果刺五加苷B、刺五加苷E分别在0.35～34.83与0.69～69.20μg.mL-1内线性关系良好,其相关系数分别为0.999 9与1.000 0;精密度RSD均为0.4%,小于2%;重复性RSD分别为1.4%与1.0%,稳定性RSD分别为3.1%与3.4%,均小于5%;平均回收率(n=9)分别为97.4%(RSD为5.5%)和102.7%(RSD为4.3%)。结论本方法操作简便,结果可靠,重复性好,可作为刺五加及其制剂的质量控制方法。

OBJECTIVE To develope a HPLC method for quantitative determination of eleutheroside B and eleutheroside E in Acanthopanax senticosus. METHODS The separation of eleutheroside B and elentheroside E was performed on an Agilent Zorbax SB- C18 column （4. 6 mm × 250 mm,5 μm） by gradient elution with 0. 5% aqueous phosphoric acid and acetonitrile as the mobile phase. The flow rate was 1.0 mL · min- 1 at 25 ℃, and the detection wavelength was set at 220 nm. RESULTS The linear ranges of eleutheroside B and eleutheroside E were 0. 35 - 34. 83 and 0. 69 - 69. 20 μg . mL-1, respectively, and the correlation coefficient were 0. 999 9 and 1. 000 0, respectively. The variations for intra- and inter-day precision were less than 3.1% and 3.4% ,and the accuracy （ n = 9） for eleuthereside B and eleutheroside E were 97.4% （ RSD = 5.5% ） and 102. 7% （ RSD = 4. 3% ）, respectively. CONCLUSION This method is simple, accurate,reliable and reproducible for the quality control of Acanthopanax senticosus.