高糖对肾小管上皮细胞ACE／ACE2表达的影响

Effects of high glucose on the expression of angiotensin-converting enzyme/angiotensin converting enzyme 2 in human proximal tubular cells

体外培养的HK-2细胞分为正常对照组（NG组）、高糖组（HG组）、渗透压对照组（MG组），检测细胞ACE、ACE2 mRNA和蛋白表达及细胞上清液血管紧张素Ⅱ的浓度。结果在NG组，正常培养的HK-2细胞即存在ACE、ACE2 mRNA及蛋白表达。在HG组HK-2细胞，ACE mRNA及蛋白表达较NG组升高，而ACE2 mRNA及蛋白表达降低。在HG组血管紧张素Ⅱ水平较NG组显著升高。结果表明高糖可促进体外培养的HK-2细胞ACE表达、抑制ACE2表达，进而促进血管紧张素Ⅱ合成。

HK-2 cells cultured in vitro were divided into three groups ： normal glucose group （ NG）, high glucose group （ HG）, and mannitol group （MG）. The expression of angiotensin-converting enzyme （ACE） and ACE2 mRNA in HK-2 ceils was detected. The concentration of angiotensin Ⅱ （ Ang Ⅱ ） in the culture medium was detected. The mRNA and protein expression of ACE and ACE2 existed in normal cultured HK-2 （ NG group）. In comparison with NG group, the mRNA and protein expressions of ACE in HG group increased significantly （ P 〈 0. 01 ）, and the expression of ACE2 mRNA decreased significantly（ P〈0. 01 ）. The level of Ang Ⅱ in HG group was significantly higher than in NG group（ P〈0.05 ）. The result show that high glucose may induce ACE expression and inhibit ACE2 expression, then promote synthesis of Ang H in proximal tubular cells.