石墨炉原子吸收光谱法直接测定血中铅

Direct Determination of Lead in Blood by Graphite Furnace Atomic Absorption Spectrometry

[目的]建立稀硝酸稀释血样,石墨炉原子吸收直接测定血铅的方法。[方法]采用1.0%硝酸10倍稀释血样,10μL进样,标准加入法制备标准曲线,直接检测血铅。[结果]在实验条件下,血铅浓度在0.0～100.0μg/L之间存在线性关系(r=0.9992)。铅标准溶液25.0、50.0、75.0μg/L分别平行测定6次,其相对标准偏差为1.28%～2.74%。血样中加入铅标准溶液25.0、50.0、75.0μg/L,测得血铅回收率为95.7%～102.0%。[结论]该法灵敏度高、正确度好,操作简便快速,适宜血液中铅的测定。

[ Objective ] To establish a direct blood lead determination methodology by graphite furnace atomic absorption spectrometry using nitric acid treated samples. [ Methods ] Blood samples were pre-treated by 0.1% nitric acid and injected to a graphite furnace atomic absorption spectrometer. [ Results ] The linear range of proposed determination methodology was 0.0-100.0 μg/L （r= 0.999 2）. The standard solutions containing lead at 25.0, 50.0 and 75.0 μg/L were tested six times under the same conditions. The relative standard deviations were 1.28%-2.74% andthe average recoverty-ratios were 95.7%-102.0% for blood samples adding to the same series standard lead solutions. [ Conclusion ] The direct blood lead determination method shows high sensitivity, good linear range and convenience in operation.