Cu^(2+)、Cd^(2+)和Cr^(6+)对斑马鱼联合毒性作用和生物预警的研究

Study on Joint Toxicity and Biological Early Warning of Cu^(2+), Cd^(2+) and Cr^(6+) Using Zebrafish

为了研究重金属对水生生物的联合毒性作用,以斑马鱼为受试生物,采用半静态法,在研究Cu2+、Cd2+和Cr6+对斑马鱼单一毒性的基础上,以获得的Cu2+、Cd2+和Cr6+的96h-LC50数据,利用等效应曲线法研究了Cu-Cd、Cu-Cr和Cd-Cr的联合毒性作用,并综合分析环境毒理学指标的测定结果,考察利用斑马鱼作为水质监测指示生物的可行性。结果表明:Cu2+、Cd2+和Cr6+对斑马鱼的96h-LC50分别为0.296、17.72和64.64mg·L-1,毒性顺序为Cu2+>Cd2+>Cr6+,其中Cu2+为高毒物质,Cd2+和Cr6+为低毒物质;Cu-Cd、Cu-Cr和Cd-Cr对斑马鱼毒性作用类型分别为协同作用、拮抗作用和协同作用。CAT活性与Cu2+、Cd2+和Cr6+浓度均具有显著的剂量-效应关系,因此斑马鱼可以作为监测重金属污染的指示生物。

To study the joint toxic effect of heavy metals on the aquatic species, the toxicity of single Cu2＋, Cd2＋ and Cr6＋ was studied using zebrafish by semi-static test, and based on the obtained 96 h-LC50 the joint effect of Cu-Cd, Cu-Cr and Cd-Cr to zebrafish was investigated using the equivalent curve model. Analysis of toxicological indices was performed to study the possibility of using zebrafish as a biological indicator in water quality monitoring. The results showed that the 96 h-LC50 of Cu2＋, Cd2＋ and Cr6＋ was 0.296, 17.72 and 64.63 mg·L-1, respectively. The toxicity followed the order of Cu2＋Cd2＋Cr6＋. Cu2＋ was high toxic substance, while Cd2＋ and Cr6＋ were low toxic substances. The types of joint effects of Cu-Cd, Cu-Cr and Cd-Cr to zebrafish were synergistic effect, antagonistic effect and synergistic effect, respectively. The activities of CAT and the concentrations of Cu2＋, Cd2＋ and Cr6＋ showed obvious dose-response relationship, respectively. So zebrafish can be used as a biological indicator for monitoring heavy metal pollution.