兔抗hCG CP22抗血清体外抑制肿瘤细胞生长的研究

Inhibition of rabbit anti-hCG CP22 to tumor cells in vitro

目的:探讨抗原抗体反应检验若干人肿瘤细胞分泌β-hCG情况,和抗hCGCP22抗血清体外抑制肿瘤的作用。方法:采用细胞免疫荧光染色测定5个肿瘤细胞株是否表达β-hCG,用CCK-8试剂盒检测CP22抗血清(AS CP22)和β-hCG羧基端37肽(CTP)抗血清(AS CTP)体外抑制肿瘤细胞生长的光密度。结果:以AS CP22为探针和AS CTP为阳性对照,体外培养的人胰腺癌BXPC-3、人卵巢癌HO-8910和人结肠癌Lovo细胞膜上都能观察到绿色荧光,提示肿瘤细胞均表达β-hCG亚单位,而人前列腺癌PC-3和Du-145细胞则未检测到绿色荧光;与正常兔血清(NS)比较,AS CP22和AS CTP均可显著的体外抑制BXPC-3和HO-8910细胞生长,P<0.001。AS CP22抑制BXPC-3和HO-8910肿瘤细胞的作用都强于AS CTP,P<0.05。结论:人胰腺癌BXPC-3、人卵巢癌HO-8910和人结肠癌Lovo细胞在体外培养的情况下均表达β-hCG亚单位,且它们的体外生长都能被兔抗hCG嵌合肽(CP22)抗血清体外抑制。

OBJECTIVE： To observe whether several human tumor cells secret β-hCG subunit and the serum against hCG chim- eric peptide （CP22） inhibits tumor cell proliferation in vitro. METHODS： Whether five strains of human tumor cells express β-hCG were determined using immunofluorescence staining, and the optical density of tumor cells growth inhibited by anti-CP22 an- tiserum （AS CP22） and anti- β-hCG C-terminal 37 residue synthet- ic peptide serum （AS CTP） in vitro was determined using CKK-8 kit. RESULTS.. Using AS CP22 as probe and AS CTP as positive control, the green immunofluorescence on human pancreatic （BX- PC-3）, ovarian （HO-8910） and colorectal （Lovo） cells were found in vitro, suggesting that these tumor cells expressed β-hCG subunit, whereas that did not observed on prostate tumor （PC-3 and Du-145） cells. Compared with normal rabbit serum, the growth of BXPC-3 and HO-8910 cells in vitro could be inhibited notably by rabbit AS CP22 and AS CTP （NS, P〈0. 001）. Also, the inhibi- tory effects of AS CP22 to BXPC-3 and HO-8910 cells were better than those of AS CTP （P〈0.05） when using four different dilutions of both rabbit antisera. CONCLUSION： In five strains of human tumor cells selected in this study, the BXPC-3, HO-8910 and Lovo cells express β-hCG subunit in vitro and their growth in vitro can be inhibited by the rabbit serum to hCG CP22.