气质联用法对黄精炮制前后挥发性成分的分析

Comparative analysis of volatile fractions in Polygonati Rhizoma and its processed products by GC-MS

目的:研究炮制对黄精挥发性成分的影响。方法:采用水蒸气蒸馏-气质联用法(SD-GC-MS)和吹扫捕集-热脱附气质联用法(P&T-TD-GC-MS)分别对黄精炮制前后的挥发性成分进行分析。结果:SD-GC-MS鉴定出黄精挥发性成分51种,P&T-TD-GC-MS鉴定出11种化合物。炮制后黄精挥发性成分在含量和组成上均明显降低。结论:本研究从黄精炮制前后挥发性成分变化的角度,为进一步探讨黄精炮制的减毒机制提供了科学依据。

Objective： To conduct comparative study on the volatile components from Polygonati Rhizoma during processing.Method： Volatile oil was obtained from Polygonati Rhizoma by steam distillation（SD）.Volatile components were concentrated by a purge and trap-thermal desorption（PT-TD） method,and analyzed with gas chromatography-mass spectrometry（GC-MS）,which were comparative by analyzed with the method of SD-GC-MS simultaneously.Result： The change in quantity and quality of volatile components in pre and post processed Polygonati Rhizoma were observed.Fifty-one compounds were checked out with SD-GC-MS,while 11 compounds with PT-TD-GC-MS.Conclusion： This study is useful to illustrate the mechanism of decreasing toxicity and stimulating components after being processed.