溪黄草的化学成分研究

Studies on chemical constituents of Rabdosia serra

目的:对溪黄草的化学成分进行研究。方法:利用萃取,硅胶、凝胶和反相硅胶柱色谱以及高效液相色谱法进行分离和纯化,采用波谱学技术(1D和2D NMR,MS,IR等)进行结构鉴定。结果:分离并鉴定出6个化合物,分别是ent-1α,7α,14β,20-tetrahydroxy-11,16-kauradien-15-one(1),kamebakaurin(2),dihydrokamebakaurin(3),rabdoinflexin A(4),胡萝卜苷(5),β-谷甾醇(6)。结论:其中化合物1为新化合物,化合物3为首次从该植物中分离得到。

Objective： To study the chemical constituents of aerial part of Rabdosia serra.Method： The compounds were isolated by extraction,coloum chromatography over silica gel and ODS,and preparative HPLC.Their structures were identified by various spectroscopic methods including MS,IR,1D and 2D NMR data.Result： Six compounds were isolated from R.serra and were characterized as ent-1ɑ,7ɑ,14β,20-tetrahydroxy-11,16-kauradien-15-one（1）,kamebakaurin（2）,dihydrokamebakaurin（3）,rabdoinflexin A（4）,daucosterol（5）,and β-sitosterol（6）.Conclusion： Compound 1 is a new compound,and coumpound 3 was obtained from this plant for the first time.