RP-HPLC同时测定高挂草中4种酚酸类成分的含量

Determination of atranol,lecanorin,ethyl orsellinate and methyl orsellinate in Usnea diffracta by RP-HPLC

目的:建立同时测定高挂草中atranol,茶渍酸,苔色酸乙酯,苔色酸甲酯含量的反相高效液相色谱法。方法:Kromasil C18色谱柱(4.6 mm×250 mm,5μm);以1%冰醋酸为流动相A,乙腈为流动相B,梯度洗脱;检测波长为280 nm;流速1.0 mL.min-1;柱温25℃。结果:Atranol,茶渍酸,苔色酸乙酯,苔色酸甲酯在测定范围内均表现出良好的线性;平均回收率分别为101.9%,96.3%,95.3%,102.9%,RSD分别为2.3%,1.9%,1.9%,1.9%。结论:本方法操作简单、快捷、重现性好、准确率高,可用于同时测定裕固族草药高挂草中atranol,茶渍酸,苔色酸乙酯,苔色酸甲酯的含量。

Objective： To develop a RP-HPLC method for determining the contents of atranol,lecanorin,ethyl orsellinate and methyl orsellinate in Usnea diffracta.Method： A Kromasil-C18 column（4.6 mm×250 mm,5 μm） was used at 25 ℃ with the mobile phases of acetonitrile-1% acetic acid in a gradient manner.The flow rate was set at 1.0 mL·min-1.The detection wavelength was 280 nm.Result： The correlation coefficients of atranol,lecanorin,ethyl orsellinate,and methyl orsellinate were higher than 0.999.Recoveries were from 102.9% to 95.30%;with RSD from 2.3% to 1.9%.Conclusion： The method is quick,simple and repeatable for simultaneous determination of atranol,lecanorin,ethyl orsellinate and methyl orsellinate in U.diffracta.