中国成都汉族与泰国人群Nogo蛋白基因遗传多态性研究

Genetic Polymorphism of Nogo Gene in Han Chinese of Chengdu and Thai Populations

轴索生长抑制因子(Neurite growth inhibitor,Nogo)在中枢神经系统中主要发挥抑制轴突再生的作用,其基因TATA和CAA插入缺失多态性与慢性精神分裂症发病风险有关,在不同种族间RTN4基因型及等位基因频率分布的存在差异.该研究将采用聚合酶链反应和聚丙烯酰胺凝胶电泳方法检测205名中国成都汉族人和101名泰国人RTN4基因多态性,比较两组人群RTN4基因TATC和CAA插入缺失多态性分布情况,并与不同种族人群研究结果进行比较.研究发现在中国成都地区汉族人群中,RTN4基因(TATC)2(TATC)2基因型频率为10.2%,明显低于泰国人群(21.8%),差异具有统计学意义(P=0.006);(TATC)2等位基因在成都汉族人群中占34.9%,明显低于泰国人群(45.0%),差异具有统计学意义(P=0.015).CAA插入缺失基因型和等位基因频率在成都汉族人群和泰国人群中差异均无统计学意义(P>0.05).此外,中国成都地区汉族人群等位基因(TATC)2频率(34.9%)明显低于法国加拿大人(45.0%)和突尼斯人(49.0%),差异具有统计学意义(P<0.05);等位基因(CAA)2频率(66.8%)明显高于法国加拿大人(53.0%)和巴西人(37.8%),差异具有统计学意义(P<0.05);结果表明RTN4基因TATA和CAA插入缺失多态性在不同种族间的分布存在明显差异.

Neurite growth inhibitor （Nogo） play a major role in inhibiting axonal regeneration in the central nervous system. TATA and CAA insertion deletion polymorphism is related to risk of chronic schizophrenia. RTN4 genotype and allele frequency distribution is differences in various ethnic. The genotypes and allele frequencies of Nogo gene TATC deletion and CAA insertion were analyzed in 205 healthy Han Chinese of Chengdu and 101 Thai individuals using polymerase chain reaction-polyacrylamide gel electrophoresis strategy and DNA sequencing. The frequency of the （TATC）2 （TATC）2 genotype of TATA deletion was 10.2% in Hart Chinese of Chengdu, which was significantly lower than that in Thai population. The frequency of the （TATC）2 allele was 34.9% in Han Chinese of Chengdu, which was significantly lower than that in Thai population as well. However, there was no significant difference in the genotype and allele frequency of CAA insertion between Hart Chinese of Chengdu and Thai population. Additionally, the frequency of （TATC）2 allele was significantly lower than that in French-Canadian and Tunisian, which was 45.0% and 49.0% respectively. The frequency of （CAA）2 allele was 66.8%, which was significantly higher than that in French-Canadian and Brazilian （53.0% and 37.8%, respectively）. The results suggested that TATC deletion and CAA insertion of Nogo gene in diverse populations is significantly different.