小鼠肝癌与肝正常细胞系ST3Gal和ST6Gal家族mRNA表达差异研究

Different Expression of ST3Gal and ST6Gal mRNA Between Mouse Hepatocellular Carcinoma Cell Lines

探讨肝癌细胞系Hepa1-6与肝正常细胞系BNL CL.2唾液酸糖基转移酶ST3Gal和ST6Gal家族mRNA表达的差异以及与细胞膜唾液酸含量的关系,采用RT-PCR方法检测ST3Gal唾液酸转移酶家族6个成员以及ST6Gal唾液酸转移酶家族2个成员mRNA表达差异,用凝集素芯片检测细胞膜表面唾液酸表达情况,结果显示:与正常细胞系BNL CL.2相比,hepa1-6细胞内唾液酸转移酶ST3GalⅠ、ST3GalⅣ、ST3GalⅥ呈现高表达,ST3GalⅤ低表达,ST3GalⅡ、ST3GalⅢ表达无显著性差异,两细胞系内均为检测出ST6GalⅠ表达,ST6GalⅡ表达无显著差异;hepa1-6细胞膜α2-3和α2-6连接唾液酸含量均显著增加;提示ST3GalⅠ、ST3GalⅣ、ST3GalⅤ、ST3GalⅥ可能与肝癌发生过程相关,ST3GalⅠ、ST3GalⅣ、ST3GalⅥ可能与肝癌细胞膜α2-3唾液酸含量增加相关,ST6Gal家族对细胞膜α2-6连接唾液酸含量增加无贡献.

To investigate the different expression of sialytransferase between liver cancer cell line Hepal-6 and normal cell line BNL CL.2, the mRNAs of ST3Gal family 6 members and ST6Gal family 2 members were detected by RT-PCR. The sialic acid levels on cell membrane surface were analyzed by lectin array. Compared with normal cell line, BNL CL.2, ST3Gal Ⅰ , ST3Gal Ⅳ, ST3Gal Ⅵ were identified highly expressed, ST3Gal V was lowly expressed, ST6Gal Ⅰ was not detected and there was no statistical difference between two cell lines in ST6Gal Ⅱ. The results indicate that ST3Gal Ⅰ , ST3Gal Ⅳ, ST3Gal Ⅴ, ST3Gal Ⅵ may play roles in liver cancer formation and ST3Gal Ⅰ , ST3Gal Ⅳ, ST3Gal Ⅵ are responsible for the increased level of of 2-3 sialic acid on liver cancer cell membrane surface, ST6Gal family members have no correlation with the increased level of a 2-6 sialic acid on liver cancer cell surface.