CD40和CD86在慢性阻塞性肺疾病患者诱导痰中的变化及意义

Significance of CD40 and CD86 in the Induced Sputum of Patients with Chronic Obstructive Pulmonary Disease

目的检测慢性阻塞性肺疾病(COPD)患者诱导痰中CD40和CD86水平的变化,分析CD40和CD86在COPD发病机制中的作用。方法以COPD患者为研究对象,并以无COPD者健康人作为对照,采用沙丁胺醇加高渗盐水超声雾化吸入方法诱导排痰,以流式细胞技术(FCM)检测痰液中CD40及CD86水平。同时,常规方法测定肺功能FEV1%及FEV1/FVC值。结果与正常对照组和吸烟无COPD组相比,所有COPD患者,无论GOLD分级水平高低,CD40和CD86表达水平均明显升高,差异有统计学意义(P<0.01)。COPD患者中,随着GOLD分级水平增加和FEV1%降低,CD40和CD86水平逐渐降低,与FEV1%呈明显负相关。结论 COPD患者气道内DCs数量明显增加,且与COPD病情的严重程度呈正相关。

Objective The function of CD40 and CD86 were investigated in the pathogensis of chronic obstructive pulmonary disease（COPD） through the change of CD40 and CD86 in the induced sputum of patients with COPD.Methods The cells count determined by CD40 and CD86 were analyzed with flow cytometry in the sputum collected from COPD Groups,Cigarette Smoke Groups and Control Groups.FEV1% and FEV1/FVC were determined,and the correlation between DCs and FEV1% was analyzed in all subjects.ResultsFlow cytometric analysis of CD40 and CD86 in the GOLD I^IV of COPD groups showed the upregulation of both CD40 and CD86,compared with CD40 and CD86 in Cigarette Smoke Groups and Control Groups.There was negative correlation between CD40 and FEV1%（r=-0.913,P0.05）.There was also negative correlation between CD86 and FEV1%（r=-0.893,P0.05）.Conclusion The significantly upregulation of CD40 and CD86 in the patients with COPD and the positive correlation between CD40,CD86 and the stage of COPD showed that CD40 and CD86 maybe contribute to the inflammatory reaction of COPD.