吗啡依赖大鼠皮质内BDNF和即早基因c-fos的表达

Expression of BDNF and immediate-early gene c-fos in cortex of rats after chronic morphine treatment

目的建立大鼠条件性位置偏爱(CPP)模型,探讨脑源性神经营养因子(BDNF)和即早基因c-fos在大鼠吗啡精神依赖中所起的作用。方法大鼠连续6d腹腔注射吗啡mg/kg.d-1,诱导吗啡CPP形成。免疫组化法测定大鼠皮质内BDNF和c-fos的表达。结果经6d吗啡训练后,吗啡组在伴药侧的停留时间较对照组显著增加(P<0.01)。吗啡依赖组大鼠皮质内BDNF和c-fos阳性细胞数、平均光密度值均显著高于对照组(P<0.01,P<0.01)。结论吗啡诱导CPP形成,BDNF和c-fos在慢性吗啡处理大鼠皮质内的表达上调,表明二者可能参与吗啡精神依赖的形成。

Objective To establish the rat model of morphine-induced conditioned place preference （CPP）, and to investigate the roles of brain-droved neurotrophic factor（BDNF）and c-los in morphine psychological dependence. Methods Morphine was administrated via subcutaneous injection at mg/kg · d i for 6 days to induce morphine induced conditioned place preference. The expression of BDNF and c-fos in the cortex were estimated by immunohistochemistry. Results The time spent in morphine-paired side was significantly increased in morphine-group trained after morphine treatment for 6 days, Compared with control group,P〈0.01. The mean optical density and positive cell of the BDNF and c-fos in cortex significantly increased in morphine group（ P〈0.01, P〈0.01）. Conclusion Morphine can induce the CPP in rats. The expression of BDNF and c fos can increase in rat cortex after chronic morphine treatment, which suggests that BDNF and c-fos may be related to the development of morphine dependence.