HPLC法测定半夏中鸟苷的含量

Determination of Guanosine in Pinellia ternate Breit by HPLC

目的建立半夏中鸟苷的含量测定方法。方法采用高效液相色谱法,色谱柱为Agilent Zorbax SB-C18（4.6×250mm,5μm）,流动相为甲醇：0.01%醋酸（3∶97）;检测波长254nm;流速为1.0mL.min-1。结果本法精密度高,稳定性好,鸟苷溶液在0.00411~0.0411mg.mL-1的浓度范围内具有良好的线性关系（r=0.9998）,平均加样回收率为103.26%,RSD%为2.46（n=6）。结论本方法简便,准确,重现性好,可作为半夏的含量测定方法。

OBJECTIVE To establish a method for determination of in Pinellia ternate Breit.METHODS Using a HPLC method with Agilent Zorbax SB-C18（4.6×250mm,5μm） column;the mobile phase was methanol 0.01% ethanoic acid（3∶97）;the detection wavelength was set at 254nm;flowrate was 1.0mL·min-1;The precision and stability were fine1the linean range within 0.00411~0.0411mg·mL-1,r=0.9998.The average rocoveries of dihydromyricetine（n=6） was 103.26% with RSD 2.46%.CONCLUSION The method was simple,accurate and reproducible,and can be used to determine theGuanosine in Pinellia ternate Breit.