甘蓝型油菜硫苷含量性状的SSR连锁标记

SSR-based Genetic Linkage Analysis of Glucosinolate Content in Brassica napus L

以硫苷性状差异很大而其它品质性状和农艺性状相近的油菜品系967H和967L及其F1、F2植株为材料,通过建立高硫苷和低硫苷亲本基因池来筛选表现差异的SSR引物,利用差异SSR引物对F2代植株硫苷含量性状进行标记分析,确定其与硫苷性状相连锁的关系。结果表明,SSR引物CB10364与硫苷性状连锁,连锁相关性达极显著水平(F=46.32,p<0.001);单因素方差分析表明,标记CB10364对低硫苷性状的贡献率为34.36%。

The objectives of this study were to identify SSR markers for glucosinolate traits to facilitate molecular marker-assisted breeding in Brassica napus L. Two parents 967H and 967L, which have similar quality and agronomic traits except for the glucosinolate content, and their Ft, F2 plants were investigated in this research. Two DNA bulks were constructed by mixing the DNAs of high glucosinolate plants and low glucosinolate plants in the F2 group using BSA method. To find molecular markers linked to glueosinolate content, SSR primers were used in screening parents and DNA bulks. Polymorphic SSR primers were then used in analyzing the linkage relationship between molecular markers and glucosinolate content in F2 group. The results showed that SSR primer CB10364 linked with the glucosinolate trait and reached the level of highly significant （F=46.32, P〈0.001）. One-way analysis of variance indicated that the glucosinolate trait of 34.36% is contributed by the CB 10364 marker.