补充扇贝肽对运动大鼠停训后萎缩骨骼肌细胞Caspase-3活性的影响

Effects of scallop peptides supplementation on the activity of caspase-3 of atrophic muscle after detraining in rats

目的:通过测试萎缩骨骼肌细胞Caspase-3、SOD活性和MDA含量,观察补充扇贝肽对运动大鼠停训导致骨骼肌萎缩后肌细胞Caspase-3活性及其抗氧化能力的影响,研究扇贝肽对停训后骨骼肌萎缩的干预作用。方法:40只SD雄性大鼠以15 m/min的速度,持续性水平跑台训练30 min开始进行递增负荷运动,运动6 d/周,休息1 d/周,速度递增3 m/min/周,时间递增30 min/周,运动3周后即刻处死10只大鼠为基础对照组,其余大鼠随机分组为补充安慰剂阴性对照组、补充牛乳阳性对照组和补充扇贝肽实验组,30只大鼠左侧后肢实施石膏固定模拟停训模型,并进行扇贝肽补充实验。实验组和对照组所有动物每天分别进行灌胃15%扇贝肽饮料2mL、等量的安慰剂和牛乳。结果:发现补充扇贝肽可显著减低停训后萎缩骨骼肌细胞MDA含量增加,增加SOD活性,提高细胞内抗氧化能力,可显著抑制停训后萎缩骨骼肌细胞Caspase-3的活性,从而抑制骨骼肌收缩蛋白和结构蛋白的降解,促进萎缩骨骼肌的恢复。

Objective： The effects of scallop peptides supplementation on the activity of caspase -3 and SOD and the content of MDA in skeletal muscle after detraining were investigated. To study the effects of scallop peptides supplementation on muscular atrophy after detraining in rats. Methods ： 40 male Sprague -Dawley rats ran 30 minutes at speed of 15 m/min , exercise 5 day/week and rest 1 day/week in the first week. In the second week, rats ran 60 minutes at speed of 18 m/min, exercise 5 day/week and rest 1 day/week and ran 90 minutes at speed of 21 m/min, exercise 5 day/week and rest 1 day/week in the third week. After sports training for 3 weeks, Ten rats were killed as baseline group. The 30 rats were randomly divided into 3 groups as placebo, milk and SPs supplemented, Then the left hind limbs of the rats were fixed by plastic gypsum for 3 weeks. Rats in experimental groups were fed 2ml beverage containing 15% SPs, and rats in control were fed 2ml placebo and milk respectively. Results： The results showed that After SPs supplementation, the activity of caspase -3 decreased obviously, and the antioxidant capacity increased obviously in atrophic muscle in raes.