HCV、HBV、HIV间合并感染患者血清IL-18、VEGF、TGF-β_1的含量变化

Change in serum IL-18,VEGF,and TGF-β_1 value in HCV/HBV/HIV coinfection patients

目的探讨乙型肝炎病毒(HBV)、丙型肝炎病毒(HCV)、人免疫缺陷病毒(HIV)间合并感染患者血清白细胞介素(IL)-18、血管内皮生长因子(VEGF)、转化生长因子β_1(TGF-β_1)的表达及临床意义。方法采用酶联免疫吸附试验(ELISA)法检测HBV、HCV、HIV间合并感染以及单纯HBV或HCV感染患者血清IL-18、VEGF、TGF-β_1含量,同时检测合并感染患者肝功能生化指标血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、γ-谷氨酰转肽酶(GGT)含量,并分析它们与IL-18、VEGF、TGF-β_1的相关性;另从健康体检人群中随机抽取30例作对照组进行IL-18、VEGF、TGF-β_1检测,并进行统计学分析。结果 HBV、HCV、HIV间合并感染患者血清IL-18、VEGF、TGF-β_1含量变化与性别无相关性(P>0.05);比单纯HBV和HCV感染以及正常对照组均显著升高(P<0.01),其中IL-18、VEGF、TGF-β_1值以HBV+HCV+HIV感染模式最高[分别为(312.44±45.24)pg/L、(326.43±51.24)pg/mL、(283.51±49,27)μg/L],其次为HCV+HIV感染模式[分别为(224.32±34.37)pg/L、(257.72±47.72)pg/mL、(204.11±43.28)μg/L],HBV+HCV感染模式最低[分别为(129.44±27.62)pg/L、(147.67±41.22)pg/mL、(120.47±30.23)μg/L],三者间比较,差异有统计学意义(均P<0.01)。HBV、HCV、HIV间合并感染患者血清IL-18、VEGF、TGF-β_1水平与相应患者肝功能生化指标ALT、AST、GGT均呈正相关(r值分别为:0.667、0.652、0.672;0.643、0.618、0.623;0.712、0.673、0.705)。结论检测血清IL-18、VEGF、TGF-β_1含量,对HBV、HCV、HIV间合并感染患者病情的合理评价有重要意义。

Objective To study the express and clinical significance of serum intcrleukin-18 （IL-18）, vascular endothelial growth factor （VEGF） and transforming growth factor -β1 （TGF-β1 ） in HBV/HCV/HIV co-infection pa- tients. Methods Serum IL18, VEGF and TGF-β1 levels in patients with HBV/HCV/HIV co-infection or simple HBV or HCV infection were detected with ELISA method, and liver function biochemical indicators of alanine transaminase（ALT）, aspartate aminotransferase （AST）, gamma glutamyl transferase （GGT） levels were simultaneously detected , correlation between these indicators and IL-18, VEGF, TGF-β1 was analyzed 30 healthy people were randomly selected as control group , statistical analysis was made. Results Serum IL-18, VEGF, and TGF-β1 values in patients with HBV/HCV/HIV co-infection were not associated with gender （P〉0. 05） , which were significantly higher than that of simple HBV or HCV infection and normal control group （P〈0. 01 ）,and was the highest in patients with HBV ＋ HCV ＋ HIV co-infection （IL-18, VEGF, TGF-β1 levels were [312. 44 ± 45. 24]pg/L, [326. 43± 51.24] pg/mL and [283. 51 ± 49. 27]μg/L, respectively） , followed by HCV ＋ HIV co-infection, which were （[224. 32 ± 34. 37]pg/L,[257. 72 ±47. 72]pg/mL and [204. 11 ± 43.28]μg/L, respectively）, HBV＋ HIV co-in-fection were （E129. 44 ±27. 62]pg/L, [147. 67±1. 22μg/mL and [120. 47 ± 30. 23]μg/L respectively）. In patients with HBV/HCV/HIV co-infection , serum IL-18, VEGF, and TGF-β1 levels were positively correlated with ALT,AST, and GGT （r value was 0. 667, 0. 652, 0. 672; 0. 643, 0. 618. 0. 623; 0. 712, 0. 673 and 0. 705, respectively）. Conclusion Detection of serum IL-18,VEGF,and TGF β1 levels are important to evaluate the status of patients with HBV/HCV/HIV co-infection.