二苯乙烯对2型糖尿病大鼠降血糖作用的实验研究

Effect of Stilbene on Lowering Blood Glucose of Rats with Type 2 Diabetes

目的探讨二苯乙烯对2型糖尿病降血糖作用。方法50只Wistar雄性大鼠随机分为模型组（n=40）和对照组（n=10）。模型组大鼠口服脂肪乳20ml／kg，连续40d，分别在第4l、42天时模型组大鼠腹腔注射链脲佐菌素120mg／kg、100mg／kg，各1次。建立模型后，将模型组大鼠随机分为4组，每组10只。分别灌胃给予二苯乙烯500mg／kg、200mg／kg、100mg／kg和二甲双胍100mg／kg。全自动生化分析仪测定血糖、血脂水平；分光光度计测肝糖元含量。结果建模后，大鼠的血糖（Glu）、甘油三酯fTG）、总胆固醇（TC）、低密度脂蛋白（LDL．c）、高密度脂蛋白（HDL．c）水平分别升高140．89％、25．77％、275．52％、105．54％和31．25％（P〈0．01）；给予模型组大鼠二苯乙烯20d后，血糖和血脂水平比给药前降低（P〈0．05），肝糖元含量比对照组增加8．06％～16．17％。结论二苯乙烯具有降低2型糖尿病大鼠血糖和血脂、增加肝糖元含量的作用。

Objective To study the effect of stilbene on blood glucose of rats with type 2 diabetes. Methods 50 male Wistar rats were randomly divided into model group （n-40） and control group （n=10）. The model group was given fat emulsion 40 days continuously with 20 ml/kg, po, and streptozotocin with 120 mg/kg on the 41st day, 100 mg/kg on the 42nd day, i.p. After the modeling, the model group was divided into 4 groups with 10 in each. They were given stilbene with 500 ml/kg, 200 ml/kg, 100 ml/kg and metformin with 100 mg/kg, ip, respectively. The level of blood glucose, blood lipid and glycogen content were determined. Results After the modeling, the blood glucose and TG, TC, LDL-C, HDL-C increased by 140.89%, 25.77%, 275.52%, 105.54% and 31.25% respectively （P〈0.01）; after stilbene was given, the level of blood glucose and lipid decreased significantly （P〈0.05）, the liver glycogen content increased significantly by 8.06% 16.17% compared to the control group. Conclusion Stilbene can decrease the concentration of blood glucose and lipid, and increase the liver glycogen content of rats with type 2 diabetes.