摘要
目的：建立23-羟基白桦酸及有关物质的RP-HPLC测定方法。方法：高效液相色谱法,色谱柱为Agilent Zorbax SB-C18（250 mm×4.60 mm,5μm）,流动相：乙腈-0.2%醋酸水溶液（62∶38）,流速为0.8 mL.min-1;检测波长204 nm。结果：23-羟基白桦酸进样量与峰面积呈良好线性关系,线性范围为0.20-4.0μg,r=0.999 9（n=5）;回收率为97.4%-104.7%（n=9）。结论：本法快速,简便,重复性好,可用于23-羟基白桦酸的含量测定和有关物质检查。
Objective： To establish an HPLC method to determine the content of 23-hydroxylbetulinic acid and its related substances.Methods： An HPLC method was developed.A Zorbax SB-C18 column was used,and the mobile phase consisted of acetonitrile and 0.2% acetic acid solution（62：38）.Flow rate was 0.8 mL·min^-1.Detection was at 204 nm.Results：The linear range was 0.20～4.0 μg,r = 0.999 9（n=5）.The average recovery was 101.9%～104.7%（n=9）.Conclusion： This reliable and reproducible HPLC analysis method offers an option in quality control of 23-hydroxylbetulinic acid.
出处
《中国野生植物资源》
2011年第4期50-52,共3页
Chinese Wild Plant Resources
基金
广东省关键领域重大突破项目(2003A30909)
广州市科技计划项目(2004Z2-E5021)
