湖北及山东地区栽培芋病毒病的田间调查及其病原的分子检测

Field Investigation of Viral Disease and Molecular Detection of Pathogenic Virus on Cultivated Taro [Colocasia esculenta(L.) Schott] in Hubei and Shangdong

对湖北及山东地区种植的芋调查发现,田间芋病毒病发生普遍,症状表现主要有花叶、羽状斑驳、畸形皱缩、褪绿斑点等。2010年9月30日对武汉蔬菜科学研究所国家种质武汉水生蔬菜资源圃种植的芋进行调查发现,以上4种症状的田间显症率分别为26.92%,16.67%,28.21%和3.85%,田间调查时未发现蚜虫等媒介昆虫。对不同来源的芋叶片样品进行芋花叶病毒（Dasheen mosaic virus,DsMV）的RT-PCR检测,各症状田间检出率为21.1%-42.9%。

Field investigation of cultivated taro [Colocasia esculenta（L.） Schott] in Hubei province were carried out.The results showed that taro viral diseases were happened widespread,with the main symptoms of mottle-leaf,pinnate mottle,deformity shrinks and chlorotic spots.The rates of 4 symptoms occurring were 26.92%,16.67%,28.21% and 3.85% when the investigation conducted in the National Aquatic Vegetables Resources Nursery of Wuhan Vegetables Research Institute on the September 30th,2010.No insect vectors such as aphid were found in the investigation.RT-PCR was implemented to detect DsMV of taro from Hubei.The results showed that the rates of 4 symptoms occurring ranged from 21.1%-42.9%.