正常兔眼后房压强及前后房压强差值的在体监测

Surveying of Pressure in Posterior Chamber and Pressure Difference Between Anterior and Posterior Chambers of Rabbit Eye in Vivo

目的 寻求一种眼后房穿刺方法,并在体测量兔眼的前、后房压强差.方法 利用高精度压力传感器与空气差压传感器,采用＂从角巩膜缘外周1～1.5mm处进针穿透巩膜,使针水平滑行于虹膜下而进入后房＂的扎针方法,实现在体连续监测正常兔眼麻醉状态下的后房压强与前后房压强差值.结果 麻醉状态下兔眼后房压强的范围在839.93～2662.48Pa;前、后房压强差范围是46.15～85.52Pa,均值为59.73Pa,变化周期为11.17min.结论 扎针方法测量兔眼后房压强及前后房压强差值对眼球损伤较小,监测到的正常兔眼后房压强及前后房压强差值均在合理可信的范围内.监测方法的可行性为青光眼前后房压强差值的在体监测提供了新的思路.

Objective To find a proper method of puncturing into the posterior chamber of the rabbit eye and to measure the pressure in posterior chamber and the pressure difference between anterior and posterior chambers in vivo. Methods With the help of the high-accuracy pressure and pressure difference sensors, the pressure in posterior chamber and the pressure difference between anterior and posterior chambers were measured continuously in normal rabbit eyes with anesthesia in vivo. The needle directly punctured into the posterior chamber at 1 to 1.5 millimeters away from the cornea-sclera limhus, penetrated the sclera, and then slided horizontally the needle underneath the iris. Results The pressure value in posterior chamber was from 839.93 Pa to 2662.48 Pa, while the pressure difference between anterior and posterior chambers was from 46. 15 Pa to 85.52 Pa with its average value 59.73 Pa and the wave period was about 11.17 min. Conclusions The puncture method for surveying the pressure of rabbit＇ s chambers does less hurt to the eye-ball and the experimental data of the pressures in posterior chamber and pressure difference between anterior and posterior chambers, are authentic and reasonable. This paper also provides a new method of in vivo detecting the pressure difference between anterior and posterior chambers for glaucoma patients.