麦冬皂苷D的分离提纯

Separation and purification of ophiopogonin D

以10 kg麦冬为原料,采用甲醇浸提法提取,经过脱脂、脱糖处理后得到18.1 g较纯的麦冬总皂苷,得率为0.181%。通过硅胶柱层析法分离纯化麦冬皂苷D,以氯仿-甲醇为洗脱剂进行洗脱,得到麦冬皂苷D样品2.31 g,其收率为12.8%,经高效液相色谱检测,分离得到的麦冬皂苷D的纯度为91.2%。结果表明,用硅胶柱层析法可快速分离出纯度较高的麦冬皂苷D。

18. 1 g ophiopogon total saponins were extracted from 10 kg Ophiopogon japonicus by methanol, the yield of which is 0.18%. Ophiopogon total saponins were separated on silica gel column using chloroform-methanol as eluting agent at different gradient and 2.31 g ophiopognin D was obtained, the yield of which is 12.8%. HPLC analysis was used to detect the purity of ophiopogonin D, the purity was not less than 91.2%. The result proved that silica gel column could quickly isolate high purity ophiopogonin D.