摘要
基于分光光度法检测技术,建立了样品中蛋白质含量的快速分析方法.利用试样中蛋白质与染色剂考马斯亮蓝G-250间的快速结合反应,生成有色结合物,在最大吸收波长610 nm处生成物吸光值与样品中蛋白质浓度成比例,线性范围0～100μg/mL,相关系数为0.997,检测时间少于2 min,可用于样品中蛋白质含量的快速测定.
Based on the spectrophotometry detection technique ,we estabilished a rapid analysis method for determining the protein content in samples. In the experiment, the protein in the samples quickly reacted with coomassie brilliant blue G-250 to generate a colored combination, the absorption value of the combination at the maximum absorption wavelength of 610 nm was proportional to the protein concentration in the samples, with the linear range of 0 to 100 μg/mL and linear coefficiency of 0.997. The method completed in 2 minutes, and could be used for the rapid determination of protein content in samples.
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2011年第4期27-29,35,共4页
Journal of Henan University of Technology：Natural Science Edition
基金
河南省重点科技攻关资助项目(102102310305)
河南省教育厅自然科学研究资助项目(2011A550002)
郑州市科技攻关资助项目(0910SGYS34370-3)
郑州市科技攻关资助项目(2010GYXM482)
河南工业大学博士基金资助项目(2009BS009)
