BPA不影响卵母细胞减数分裂相关基因Dazl的甲基化

No Effecting of BPA on DNA Methylation of Dazl Gene in Oocyte Meiosis

为了探讨环境雌激素BPA对小鼠卵母细胞减数分裂相关基因Dazl甲基化的影响,本研究通过给孕鼠饮用含有BPA的水方式使胎鼠在发育过程中接触BPA,利用重亚硫酸盐测序法,分析了胎鼠生殖嵴卵母细胞不同发育时期Dazl甲基化水平的变化。结果显示:Dazl在减数分裂期间处于低甲基化水平,无论对照组或处理组均低于10%,说明Dazl的低甲基化对维持减数分裂的正常进行有重要作用;对照组与处理组的甲基化水平相当,差异不显著,说明本研究的BPA浓度不影响卵母细胞Dazl的甲基化水平。

To investigate the effect of BPA （BisphenolA） on methylation states of meosis - specific gene Dazl （ Deleted in AZoospermia - Like gene） during gametogenesis, maternal mouse were treated by adding different concerntration of BPA in their water respectively . Then different developmental stages of fetal mouse oocytes were studied using bisulfite sequencing method to examine the methylation states of Dazl. Our results implicated the rate of methylation of Dazl kept a low level in both control and treated groups（ 〈 10% ）, suggested hypomethylation of Dazl acts an important role of in maintainance of normal meiotic process. There was no significant difference between control and treated group, indicated a negative effect of BPA on the DNA methylation of Dazl.