梨同义密码子用法的初步分析

A preliminary Analysis of Synonymous Codon Usage in Pear(Pyrus spp.)

生物体内密码子使用偏性对外源基因表达存在较大影响。本研究采用CodonW软件和高频密码子分析法,对白梨(Pyrus bretschneideri Rehd)、西洋梨(Pyrus communis Linn)、砂梨(Pyrus pyrifolia Nakai)、秋子梨(Pyrusussuriensis Maxim)4种梨蛋白质编码基因序列(Codon DNA sequence,CDS)进行了同义密码子相对使用频率(relativefrequency of synonymous,RFSC)分析,确定了梨高频密码子共23个,结果同时显示梨不同种之间在密码子使用上具有较高的一致性。

Codon usage bias in organism has an important influence on exogenous gene expression. In this study, relative frequency of synonymous codon of DNA sequence （CDS） of four different pears species, including Chinese pear （Pyrus bretschneideri）, Europear pear （Pyrus communis）, sand pear （Pyrus pyrifolia） and ussurian pear （Pyrus ussuriensis） was analyzed by CodonW and high -frequency codon analysis, and 23 high h - frequency codons were detected. The results also revealed that codon usage of different pear species were consistent with each other.