家驴基因组高度重复DNA序列的分离及其文库的构建

Separation of highly repetitive DNA for construction HR Cot library of Equus asinus

为研究家驴基因组重复序列,通过2次羟基磷灰石柱层析,分离纯化家驴Cot 0-Cot 0.1之间的基因组高度重复DNA序列,用于构建家驴基因组Cot文库,并向GenBank提交了18条重复序列,其中4条含有LINEs序列片段,4条含有SINEs序列片段,3条含有卫星序列片段,4条含有简单重复序列片段,1条含有LTR-逆转录转座子片段,2条未知功能的重复序列。

In order to investigate the highly repetitive（HR） DNA sequences among Equus asinus genome,Cot0-Cot0.1 DNA of E.asinus genome was separated and purified through two steps of hydroxypatite chromatography.Then the HR Cot library of E.asinus was constructed by gene cloning and sequencing.After the analysis and screening of the library,18 repetitive elements were identified and submitted to GenBank,including 4 LINEs,4 SINEs,3 satellites,4 simple repeats,1 LTR retrotransposon and 2 unknowns.