斜纹夜蛾核型多角体病毒对宿主子代的弱化作用

Debilitation effect of nucleopolyhedrovirus on the progeny of host Spodoptera litura

用9.44×108 OB/mL和9.44×107 OB/mL病毒液分别饲喂斜纹夜蛾(Spodoptera litura)幼虫和成虫,观察斜纹夜蛾核型多角体病毒(SlNPV)对宿主子1、2代的影响。结果表明:斜纹夜蛾4～6龄初幼虫饲毒后,F1代、F2代化蛹率分别下降了12.73%～18.59%、5.88%～10.21%,羽化率分别下降了11.31%～15.41%、5.70%～8.46%;成虫饲毒后,F1代、F2代化蛹率分别下降了14.00%～34.63%、8.33%～9.84%,羽化率分别下降了10.77%～19.61%、11.36%～12.00%;4～5龄幼虫饲毒和成虫饲毒后,F1代、F2代的蛹质量明显降低,产卵量显著下降,产卵期和雌雄成虫寿命明显缩短,但F1代、F2代幼虫历期、蛹期和产卵前期与对照差异不显著。试验结果还表明,随着宿主子代数的增加,核型多角体病毒对子代的弱化作用越来越不明显。

The experiments were conducted to determine the debilitation effect of Spodoptera litura nucleopolyhedrovirus（SlNPV） on hosts from the infected parental generation to their next two progenies through feeding the larvae with the concentration of 9.44×107 OB/mL and the adults with the concentrations of 9.44×108 OB/mL and 9.44×107 OB/mL in the laboratory.The experiment results of feeding the early 4th-6th instar larvae with SlNPV in concentration of 9.44×107 OB/mL indicated that the pupation rates of F1 and F2 progeny were respectively reduced by 12.73%-18.59%,5.88%-10.21%,and the emergence rates went down respectively done by 11.31%-15.41%,5.70%-8.46%.The experiment results of feeding the adults with SlNPV in concentrations of 9.44×108 OB/mL and 9.44×107 OB/mL showed that the pupation rates of F1 and F2 progeny were respectively reduced by 14.00%-34.63%,8.33%-9.84%,and emergence rates were respectively decreased by 10.77%-19.61%,11.36%-12.00%.After feeding the early 4th-5th instar larvae and adults with SlNPV,pupal weight and fecundity of F1 and F2 progeny were both significantly decreased,oviposition period and adult longevity were both significantly shortened,but there were was no significantly difference in larval duration,pupal stage and preoviposition period between F1 and F2 progeny.The results also indicated that the debilitation effect of SlNPV on host was weakened gradually with the increase of host progeny.