珍稀濒危植物张氏红山茶花的花发育形态及生理特性研究

Morphology and physiological characteristics of flower development in Camellia changii Ye,an endangered and rare plant

以珍稀濒危植物张氏红山茶为材料,对其花发育形态及分化过程中生理指标动态变化进行了研究。采用定株调查的方法,通过观察、统计、解剖等确定张氏红山茶盛花期花芽着生位置并划分5个发育阶段,以分光光度法测定了5个发育阶段可溶性糖、可溶性蛋白和核酸含量等的动态变化。结果表明:盛花期花芽着生位置主要在枝条顶端,占70.0%;花芽和叶芽的着生位置存在竞争现象;花芽不同发育阶段可溶性糖、可溶性蛋白、核酸含量变化趋势各不相同;在5个发育阶段,可溶性糖含量始终最高;在中期(Stage 3)可溶性蛋白含量最高。可溶性糖、核酸、可溶性蛋白三种物质含量在花芽发育末期(Stage 5)存在大致10∶2∶1的比例。

The flower development morphology and physiological characteristics of Camellia changiiYe,one of the endangered and rare species,were investigated.The formation sites and five developmental stages of flower buds were identified by observation,statistics and freehand section.Meanwhile,the dynamic changes among soluble sugar,soluble protein and nucleic acid contents were mensurated by ultraviolet absorption method.The results showed that flower buds sites were mainly located on the top of shoots,accounting for about 70.0%;formation sites of flower and leaf buds competed with each other.Flower buds at different stages had different trend among soluble sugar,soluble protein and nucleic acid contents.The soluble sugar content was the highest in all stages;soluble protein had the highest content at middle stage（Stage 3）;the rate of contents among soluble sugar,nucleic acid and soluble protein were about 10∶2∶1 in Stage 5.