HPLC法测定清热消肿口服液中绿原酸的含量

Determination of Chlorogenic acid in Qingrexiaozhong oral liquid by HPLC

目的建立HPLC法测定清热消肿口服液中绿原酸的含量。方法采用phenomenex Luna-C18柱(200 mm×4.60 mm,5μm),流动相为甲醇-1%冰醋酸(20∶80),流速为1.00 ml/min,检测波长326 nm,柱温为30℃。结果绿原酸进样量在0.163 1～1.087 2μg范围内与峰面积线性关系良好,r=1.000(n=5),平均回收率为100.40%,RSD为1.20%(n=9)。结论该方法简便、准确、可靠、重现性好,可用于该制剂的质量控制。

Objective To develop a HPLC method for determining the content of Chlorogenic acid in QingRexiaozhong Oral Liquid.Methods The phenomenex Luna -C18（200 mm×4.60 mm,5 μm） was used;the mobile phase consisted of water -1% Aceticacid（20∶80）,the flow rate of 1.00 ml/min,and the detecting wavelength was 326 nm.The column temperature was at 30 ℃.Results There was a good linearity for Chlorogenic Acid in the range of 0.163 1～1.087 2 μg,r=1.000（n=5）,and the average recovery rate was 100.40%,RSD=1.20%（n=9）.Conclusions The method was simple,accurate,reliable and reproducible,which could be available for the quality control of QingRexiaozhong Oral Liquid