4种环境污染试剂诱导蚕豆微核现象的研究

Study of Micronucleus Phenomenon of Vicia faba Induced by Four Invorment Pollution Reagent

[目的]检测4种试剂处理后蚕豆的微核情况,以此评估一些污染物的危害性。[方法]通过蚕豆根尖微核试验,根据微核出现的频率来评价环境诱变因子对蚕豆微核的损害程度,镜检计数后计算微核率。[结果]甲醛、丙烯酰胺、硝酸铅、O2衍生物可导致蚕豆根尖细胞间期细胞微核频率明显升高,但每种污染物在不同浓度会有不同程度的微核现象,且在一定浓度范围内微核率上升,浓度达到一定值时下降。[结论]丙烯酰胺、甲醛对蚕豆根尖细胞具有明显的遗传毒性效应;硝酸铅对蚕豆根尖细胞不具有明显的遗传毒性效应;SO2衍生物低浓度时对蚕豆根尖细胞有一定遗传毒性效应,高浓度效应明显。

[Objective]The aim was to study micronucleus phenomenon of Vicia faba induced by four invorment pollution reagent in order to assess some of the dangers of pollution.[Method]By the mature root tip cells of Vicia faba micronucleus in substantial progress in water pollution monitoring,micronucleus phenomenon of Vicia faba induced by four invorment pollution reagent was studied.[Result]It was found that a representative of the environmental pollutants such as formaldehyde,acrylamide,lead nitrate,SO2 derivatives could result in root tip cells of Vicia faba micronucleus frequency of interphase cells to increase significantly,but different pollutants at different concentrations varied degrees of micro-nuclear phenomenon,and in a certain range of concentration would increase the micronucleus rate,but would decline.[Conclusion] Formaldehyde and acrylamide had evident genotoxic effects on root tip cells of Vicia faba,lead nitrate had not evident genotoxic effects,and SO2 derivatives had a certain genotoxic effect at low concentration,and had evident genotoxic effects at high concentration.