359株临床分离真菌的分布及药敏分析

Distribution and Drug Susceptibility of 359 Strains Fungi Isolated from Clinical Specimens

目的探讨本院检出真菌的分布特征及药敏结果，为预防和减少院内真菌感染及临床合理使用抗真菌药物提供依据。方法利用沙保氏培养基和显色培养基进行分离培养及初步鉴定，利用ATBFUNGUS3试条进行药敏分析，将真菌鉴定和药敏结果输入WHONET5．4软件数据库，然后利用该软件进行数据分析。结果本院2008—2010年从临床分离的359株真菌，白色念珠菌占68．2％，热带念珠菌14．8％，光滑念珠菌8．6％，近平滑念珠菌6．1％，克柔念珠菌1，1％，其他真菌1．1％；对5种临床常用抗真菌药物两性霉素B、5-氟胞嘧啶、伊曲康唑、伏立康唑、氟康唑的敏感性均较高，最高为两性霉素99．6％，最低为伊曲康唑93．9％。结论临床真菌分离率呈逐年升高趋势，但没发现明显的耐药趋势，临床应加强对抗菌药物的合理使用，减少真菌感染的发生。

Objective To investigate the distribution and drug resistance of fungi isolated from clinical specimens,in order to provide a base for reduction of nosocomial infection and antifungal clinical treatment. Methods Fungi isolated were primarily identified by Sabouraud＇s Agar and CHROMagar. Antifungal agent＇s susceptibility was tested with ATB FUNGUS 3. Then input the data of distribution, drug sensitive test and patient information using software of WHONET 5.4. Results The distribution of 359 strains fungi from 2008 to 2010 is C.albicans（68.2%）, C.tropicalis（14.8%）, C.glabrata（8.6%）, C.parapsilosis（6.1%）, C.krusei（1.1%）, and othem（1.1%）. The drug sensitive test showed 359 strains fungi were all highly susceptible to amphotericin B, 5-flurocytosine, itraconazole, voriconazole, and fluconazole; The highest rate of susceptibility is amphotericin B（99.6%）, and the lowest one is itraconazole（93.9%）. Conclusion The data of fungui from 2008 to 2010 showed the tendency of Infection of fungi has increased yearly from 2008 to 2010, but drug resistance hasn＇t increased obviously. It is necessary to strengthen the control of nosocomial infection and rational use of drug.