HPLC法测定榄香烯亚微乳注射液中β-榄香烯的含量

Determination of β-elemene in Elemene Microemulsion Injection by HPLC

目的建立榄香烯亚微乳注射液中β-榄香烯的含量测定方法。方法采用HPLC法,色谱柱：迪马C18色谱柱,乙腈-水（87∶13）为流动相,检测波长210nm。结果β-榄香烯在0.1149~3.4470μg之间线性关系良好,r=0.9999,回收率为100.4%,RSD为1.0%。结论该方法操作简单、分离效果好、灵敏度高,可作为榄香烯亚微乳注射液的质量控制标准。

Objective A HPLC method for determination of β-elemene of Elemene Microemulsion Injection was established.Methods A Diamonsil C18 column was used with acetonitrile-water（87∶13） as the mobile phase.The detection wavelength was 210nm.Results The linear range was 0.1149～3.4470μg,r=0.9999.The average recovery was 100.4%,and RSD was 1.0%.Conclusion The method is simple and sensitive with good separation efficiency.It can be used in the standard of Elemene Microemulsion Injection.