^(125)I粒子联合吉西他滨对Lewis肺癌移植瘤杀伤效应的实验研究

The experimental study of lethal effect of radioactive ^(125)I particles combined with gemcitabine on mouse Lewis lung carcinoma

目的:探讨放射性125I粒子联合吉西他滨对Lewis肺癌移植瘤的杀伤效应。方法:成功建立Lewis肺癌移植瘤模型的C57/BL6雄性小鼠59只,选取移植瘤大小合适的动物模型40只并随机等分为4组:K组(n=10)为空白组,给予植入一颗空白粒子并腹腔注射生理盐水0.4ml(第1、8、15天);J组(n=10)为吉西他滨组,给予植入一颗空白粒子并按450mg/kg(按实验动物和人体表面积换算)腹腔注射吉西他滨溶液0.4 ml(第1、8、15天);IJ组(n=10)为吉西他滨联合粒子组,按治疗计划给予植入125I粒子(1.0mCi/粒)并按450mg/kg腹腔注射吉西他滨溶液0.4 ml(第1、8、15天);I组(n=10)为粒子组,按治疗计划给予125I粒子(1.0mCi/粒)并腹腔注射生理盐水0.4ml(第1、8、15天)。观察小鼠的一般情况及瘤体的生长,绘制肿瘤生长曲线;到21d取材点后处死,取出瘤体称重,计算抑瘤率、增敏系数及存活率;并进行组织病理学评价。结果:各组生长曲线明显分离,K组最高,依次为I组、J组,IJ组最低;各组取出瘤体称重并相互比较均有显著性差异(P<0.05),其中K组>I组>J组;J组、IJ组和I组抑瘤率相互比较均有显著性差异(P<0.05),其中IJ组优于J组及I组,J组优于I组;IJ组和J组存活率均与K组存活率有显著性差异(P<0.05),均明显高于K组,但与I组存活率无显著性差异(P<0.05),IJ组和J组存活率比较无显著性差异(P>0.05),I组与K组存活率比较无显著性差异(P>0.05);增敏系数:EF=NGD(规格化肿瘤生长延缓时间)/AGD(绝对肿瘤生长延缓时间)=1.45>1。结论:放射性125I粒子联合吉西他滨能显著提高对Lewis肺癌移植瘤的杀伤效应,并证实吉西他滨对125I粒子有放射增敏作用。

Objective： To investigate the lethal effect of radioactive 125I articles combined with gemcitabine on mouse Lewis lung carcinoma.Methods： We successfully established model of Lewis lung cancer on 59 C57/BL6 male mice to select a suitable animal tumor model size 40 and randomly divided into 4 groups： K group（n=10） for the control group;J group（n=10） for the gemcitabine group;IJ group（n=10） for the gemcitabine particle group;I group（n=10） for the particle group.K group was given a blank particle implantation and intraperitoneal injection of saline 0.4ml（No1,8,15days）;J group was given a blank implant particles according to 450mg/kg（body surface area by experimental animals and conversion） were intraperitoneally Gemcitabine injection solution 0.4 ml（No.1,8,15 days）;IJ group was given 125I implantation of a particle（1.0mCi） according to TPS and 450mg/kg intraperitoneal injection of gemcitabine solution 0.4 ml（No.1,8,15 days）;I group was given 125I particles（1.0mCi） and normal saline 0.4ml（No 1,8,15 days）.The general situation in mice,and tumor growth,tumor growth curve;to the point were sacrificed after 21 days,drawn,removed the tumor weight,tumor inhibition rate and survival rate sensitivity coefficient;and forhistopathological evaluation.Results：The clear separation of the growth curve,K group was the highest,followed by group I,J groups,IJ group was the lowest;removed the tumor in each group weighed and compared the differences were statistically significant（P0.05）,where K group I groupJ groupIJ group;J group,IJ group and I,the tumor inhibition rates were statistically significant differences between each other（P0.05）,IJ was better than J group and I group,J group than I group;IJ group and J and K survival rate survival rate were significantly（P0.05）,were significantly higher than the K group,but I was no significant difference in survival rate（P 0.05）,IJ J group and the survival rate difference was not statistically significant（P 0.05）,I group and K group survival difference was not statistically significant（P 0.05）; sensitizing factor： EF = NGD（normalized tumor growth delay time） / AGD（absolute tumor growth delay time） =1.451.Conclusion： 125I radioactive particles combined gemcitabine can significantly increase the Lewis lung tumor killing effect,and confirmed that gemcitabine 125I particles have radiosensitizing effect.