气相色谱-质谱法测定卷烟烟气中苯并[α]芘

GC-MS Determination of Benzo[α] Pyrene in Smoke of Cigarettes

卷烟主流烟气用剑桥滤片捕集总粒相物,然后用环己烷超声浸取总粒相物中苯并[α]芘。所得浸取液先经HP-5MS毛细管预柱(0.25mm×1.0mm,0.25μm)净化以保护分离柱和离子源,再经HP-5MS毛细管柱(0.25 mm×30mm,0.25μm)分离后,质谱测定。苯并[α]芘的质量浓度在4～640ng·L^(-1)范围内与其峰面积呈线性关系。方法的日内相对标准偏差(n=7)为2.1%,日间相对标准偏差(n=5)为2.4%。按本法分析了5个卷烟样品,所得苯并[α]芘的测定结果与国标法测定结果一致。

The total particulate matters （TPM） in mainstream smoke of cigarettes were trapped by the Cambridge filter and the benzo [α] pyrene collected was extracted with cyclohexane. The benzo [α] pyrene in the extract obtained was purified by passing through HP 5MS capillary pre-column （0.25 min× 1.0 mm, 0. 25 μm） to protect chromatographic column and ion source, and separated on HP-5MS capillary column （0. 25 mm× 30 mm, 0. 25 μm） and determined with MS detectiorL Linear relationship between values of peak area and mass concentration of benzo [α] pyrene was kept in the range of 4-640 ng · L^-1. Values of intractay RSD （n= 7） and interday （n 5） RSD found were 2. 1% and 2. 4% respectively. Five samples of cigarette were analyzed by this method, the results of benzo[α]-pyrene found were in consistency with those found by the GB standard method.