多功能柱净化-光化学柱后衍生高效液相色谱测定食品中黄曲霉毒素

Determination of aflatoxin B_1,B_2,G_1,G_2 in food by multifunctional clean-up column,post-column photochemical derivatization and HPLC

目的:研究建立了光化学柱后衍生-高效液相色谱-荧光检测器测定食品中黄曲霉毒素B1、B2、G1和G2的方法。方法:乙腈/水提取样品中黄曲霉毒素B1、B2、G1和G2,经多功能柱净化、高效液相色谱分离、光化学柱后衍生,荧光检测器测定。结果:黄曲霉毒素B1、B2、G1和G2的检出限分别为0.50μg/kg、0.15μg/kg、0.50μg/kg和0.15μg/kg,回收率为86.7%～97.2%,相对标准偏差(RSD)0.41%～2.40%。结论:该方法简便快速、灵敏度高、重现性好,满足食品中黄曲霉毒素检测的需要。

Objective：A method for determination of Aflatoxin B1,B2,G1 and G2 by using post-column photochemical derivatization-HPLC-fluorescence detector was developed.Methods：The Aflatoxin B1,B2,G1 and G2 in samples were extracted by acetonitrile/water and were cleaned up by multifunctional clean-up columns.The separation of Aflatoxin B1,B2,G1 and G2 was conducted by HPLC,the determination was carried out by fluorescence detector after photochemical derivatization.Results：The detection limits of Aflatoxin B1,B2,G1 and G2 were 0.50 μg/kg,0.15 μg/kg,0.50 μg/kg and 0.15 μg/kg respectively,the recoveries of analytes were from 86.7% to 97.2% and the relative standard deviations were 0.41%～2.40%.Conclusion：The method is simple,high sensitive and good repeatability,which is suitable for the determination of Aflatoxin in food.