蜡样芽胞杆菌及毒素引起食物中毒的实验室检测

Laboratory testing of a case of food poisoning caused by bacillus cereus infection

目的:探讨食物中毒实验室检测机制。方法:采集中毒者肛拭子、剩余食物,带回实验室进行金黄色葡萄球菌、蜡样芽胞杆菌检验。结果:4份剩余食物其中3份样品检出蜡样芽胞杆菌,并蜡样芽胞杆菌菌落总数>105 cfu/g,所有样品的细菌生化反应结果一致。并在剩余食物中检出蜡样芽胞杆菌肠毒素。肛拭子和剩余食物均未检出金黄色葡萄球菌。结论:按WS/T.82-1996诊断标准,可判定此次是由蜡样芽胞杆菌所引起的食物中毒。

Objective：Explore the mechanism of laboratory testing of food poisoning.Methods：Pathogensof Bacillus Cereus and Staphylococcus from patient＇s foods and anal swat were collected for detection.Rusults：In the 4 samples of leftover,Bacillus Cereus were found in 3 samples with aerobic plate count of Bacillus Cereus 105 cfu/g,chemical reaction of these samples were same.All these isolates have Bacillus Cereus enterotoxin.Staphylococcus was not detected from all of these.Conclusion：The Bacillus Cereus and Bacillus Cereus enterotoxin could cause the food poisoning event according to ＂WS/T.82-1996＂.