检测血清GP73对PHC诊断价值的探讨

Exploration on diagnostic significance of serum GP73 for the primary hepatocellular carcinoma

目的:通过检测血清高尔基体蛋白73(Golgi protein73,GP73)的含量,探讨GP73在原发性肝癌(PHC)中的临床诊断价值。方法:本文共收集149例血清,其中原发性肝癌57例,慢性肝炎32例,肝硬化38例,健康人22例。应用酶联免疫定量检测血清GP73含量。并对57例原发性肝癌的GP73含量与其肿瘤大小、结节数量和对29例原发性肝癌术前与术后的GP73含量进行比较。结果:原发性肝癌患者血清GP73含量显著高于慢性肝炎,肝硬化患者和正常对照组,比较有显著差异(,P均<O.01);慢性肝炎,肝硬化患者血清GP73含量显著高于正常对照组,比较有显著差异(P均<0.01)。GP73诊断PHC的敏感性达到68.42%。GP73含量与其肿瘤大小、结节数量,术前与术后均无显著差异(P>0.05)。结论:检测血清GP73含量可作为诊断PHC的—个血清标志物,与AFP联检可提高对PHC的诊断敏感性,具有良好的临床应用价值。

Objective：To investigate the level of serum Golgi protein 73（GP73） and explore the clinical diagnostic value of GP73 for the primary hepatocellular carcinoma（PHC）.Methods：The concentration of serum GP73 in HPC（n=57）,chronic hepatitis（n=32）,cirrhosis（n=38） and normal controls（n=22） were assayed with ELISA.The relationship between GP73 levels and tumor size,number of nodules in 57 HPC patients was analyzed,and the presurgical and post-surgical GP73 levels in 29 case-matched HPC patients were compared.Results：GP73 level was significantly higher in HPC patients than that in patients with chronic hepatitis,cirrhosis and normal controls（all P0.01）,and GP73 level was dramatically higher in patients with chronic hepatitis and cirrhosis than that in normal controls（all P0.01）,while GP73 levels was unrelated to the tumor size and number of nodules,and no significance was obtained between pre-and post-operation in HPC patients（all P0.05）.The sensitivity of the GP73 was 68.42% for the diagnosis of HPC.Conclusion：GP73 is a potential serum diagnostic marker for the HPC,and combining GP73 and AFP could have higher detection sensitivity than that of markers considered alone.