蜱中贝氏考克次体流行状况调查

Prevalence of Coxiella burnetii in ticks

目的:了解法国新喀里多尼亚地区蜱中贝氏考克次体的流行状况。方法:以贝氏考克次体的16SrRNA为靶基因,病原体的RNA自蜱中提取,使用荧光定量PCR技术,检测法国新喀里多尼亚地区三种蜱共456株中贝氏考克次体的流行率。结果:以30为CT值,共检测出阳性334株,阳性率73.2%。按蜱种分类:硬蜱虫2株皆为阴性;血红扇头蜱阳性163株,阳性率69.9%;边缘革蜱阳性171株,阳性率77.4%。结论:法国蜱中贝氏考克次体的流行率很高,不同种蜱阳性率不同。

Objective：To investigate the prevalence of Coxiella burnetii in ticks from France.Methods：456 ticks samples were collected from tiques in New Caledonia of France.The RNA of pathogens were extracted from the tick samples.16SrRNA was the targeted gene.Then real-time PCR was used to analyse the genes.Results：334 samples were positive in the 456 samples.The positive rate was 73.2%.There are no positive in 2 tiques.There are 163,171 positive samples in Rhipicephalus sanguiners and Dermacentor Marginatus respectively.Conclusion：The prevalence of Coxiella burnetii in French ticks was very high.The positive rates were different in different ticks.