摘要
目的:研究北京妇产医院临床感染患者分离的肠球菌属耐药情况,并对耐药基因进行检测。方法:临床分离出50株肠球菌进行菌株鉴定,采用KB法做药敏试验,PCR检测氨基糖苷类、大环内酯类、四环素类及糖肽类相关基因。结果:50株肠球菌中,共检出aac(6')/aph(2')基因16株,aph(3')-Ⅲ基因46株a,nt(6)-Ⅰ基因31株t,etM基因15株,ermB基因42株,未检出vanA和vanB基因。结论:肠球菌对临床常用的抗生素已经存在多重耐药,临床医生必须重视肠球菌所致感染的治疗,合理使用抗生素,避免耐药菌株造成医院感染的暴发流行。
Objective:To study the drug resistance from clinical infective patients and detect drug-resistant genes of Enterococcus.Methods:Strains were identified.Disc agar diffusion(K-B) was used to study the antimicrobial susceptibility and polymerase chain reaction was used to test related resistant genes about aminoglycoside,macrolide,tetracycline and glycopeptides in 50 Enterococcus strains.Results:Among all of the 50 Enterococcus strains 16 isolates were detected with aac(6')/aph(2') genes,46 isolates were detected with aph(3')-III genes,31 isolates were detected with ant(6)-I genes,15 isolates were detected with tetM genes,42 isolates were detected with ermB genes and none enterococcus strains were detected with vanA and vanB genes.Conclusion:Enterococcus are resistant to clinically common used antibiotics in different degrees.The clinical doctor should value the diagnosis and treatment of Enterococcus and adopt the different treatment projects to meet the different characteristics of Enterococcus infection.
出处
《中国卫生检验杂志》
CAS
2011年第8期2058-2059,2076,共3页
Chinese Journal of Health Laboratory Technology
基金
首都医科大学附属北京妇产医院科研基金资助课题
关键词
肠球菌
耐药性
耐药基因
Enterococcus
Antimicrobial resistantance
Drug-resistant gene