花生抗青枯病种质微卫星DNA的分离

ISOLATION OF MICROSATELLITES FROM BACTERIAL WILT RESISTANT PEANUT GERMPLASM LINES

提取抗青枯病花生材料基因组DNA,分别经Hae Ⅲ、Rsa Ⅰ酶切后,与生物素标记的6条微卫星探针杂交,富集微卫星DNA。测序分析后,得到非冗余序列180条,其中微卫星序列133条、小卫星序列47条,成功设计出141对引物。其中40对引物用于检测29份花生材料,发现5对为多态性引物,能很好地揭示野生种与栽培种四大类型的遗传差异。本研究丰富了花生微卫星引物库,为抗青枯病分子育种奠定了基础。

Genomic DNAs were extracted from bacterial wilt（BW） resistant peanut germplasm lines,digested with Hae Ⅲ/Rsa Ⅰ and screened with 6 biotin-labeled microsatellite probes to enrich microsatellite DNAs,resulting in a total of 180 non-redundant DNA sequences.Among them,133 and 47 were found to contain microsatellites and mini-satellites,respectively.A total of 141 primer pairs were designed,and 40 primer pairs were used to genotype 29 peanut accessions.Result showed that five microsatellites were polymorphic,which could differentiate the wild Arachis species and the cultivars into of four botanical types.The study provided additional microsatellites and the possible way for molecular breeding of the BW resistant peanut.