EMS诱变筛选马铃薯茎段离体耐盐变异体

SELECTION OF SALT-TOLERANT VARIANTS FROM POTATO in vitro MICRO-CUTTINGS INDUCED BY EMS

用甲基磺酸乙酯(EMS)诱变处理甘农薯2号和青薯2号试管苗茎段,进行耐盐性研究。结果表明,甘农薯2号和青薯2号试管苗离体茎段诱变处理的适宜浓度和时间组合为0.9%～1.0%+4.0h。再通过1.0%NaCl选择压进行耐盐性变异体筛选,获得了耐1.0%NaCl诱变植株。在盐胁迫条件下诱变株超氧化物歧化酶(SOD)活性增强,叶绿素和游离脯氨酸含量增加,细胞膜透性减小,表明所筛选的甘农薯2号和青薯2号诱变株比对照具有较强的耐盐性。经0.9%～1.0%浓度的EMS溶液处理4.0h可以筛选出甘农薯2号和青薯2号的耐盐变异体。

In vitro micro-cuttings of potato cv.Gannongshu 2 and cv.Qingshu 2 were used as materials to study induction effect of ethyl methyl sulfonate（EMS） on selection of salt-tolerance variants.The results showed that the optimun,EMS concentration was 0.9%～1.0% and the most suitable treated duration was 4 hours for both microcuttings.The materials were treated by EMS（0.9%～1.0% ＋ 4.0h） and then salt-tolerance variants under 1.0% NaCl stress was selected.The variants presented stronger superoxide dismutase（SOD） activity,less cell membrane permeability,higher content of free proline and chlorophyll than the control,which indicated that the variants have stronger salt-tolerance.