泰州市食品、公共场所从业人员沙门氏菌携带状况及耐药谱变化的研究

Study on Salmonella carrying situation and antibiotic resistance profile of employees in food service industry at Taizhou

目的了解泰州市食品、公共场所从业人员沙门菌的携带状况及耐药谱的变化。方法对从业人员的肛拭子标本进行增菌、分离培养,对分离的可疑菌株进行生化和血清学分型鉴定和药敏试验。结果 2008、2009、2010年度的从业人员沙门氏菌检出率分别为0.49%、0.46%、0.38%,平均检出率为0.44%,各年度检出率之间(P>0.05)无显著性差异。在菌型分布中以B群的德尔卑、阿贡纳和C群的汤卜逊沙门氏菌为主要菌型。除对头孢噻肟、头孢吡肟和头胞西丁敏感性较高外,对其余的19种抗生素均产生一定耐药性。247株沙门氏菌中有180株对2种或以上抗生素耐药,15个血清型的沙门氏菌均二重以上耐药,以鼠伤寒沙门氏菌和德尔卑沙门氏菌的多重耐药最为严重,最高达十二重。结论该市食品、公共场所从业人员沙门氏菌的携带状况近几年未发生大的改变。从业人员携带的沙门氏菌对常用的抗生素具有较强的耐药性,应继续加强从业人员健康体检的肠道致病菌检测工作,对沙门氏菌的携带者进行规范化服药,以预防食源性疾病的发生。

Objective To understand the carrying situation of Salmonella and its antibiotic resistance profile.Method The anal swabs specimens were collected from employees,and the specimens were carried out pre-enrichment and isolate culture,and then the suspected strains were typed by biochemical and serological methods and followed drug sensitive test.Results The detection rates of Salmonella were 0.49%,0.46% and 0.38% respectively in 2008,2009 and 2010,with an average of 0.44%,and there were no significant different between different years（P0.05）.According to the results of identification,the major serotypes of Salmonella were Derby and Agona which belonged to group B,and Thompson which belonged to group C.The results of drug sensitive test indicated that Salmonella had gained antibiotic resistance against 19 kinds of antibiotics except of cefotaxime,cefepime and cefoxitin.180 strains among 247 isolated Salmonella gained 2plex plus antibiotic resistance,in all,15 serotypes of Salmonella were 2plex plus antibiotic resistance,especially Typhimurium and Derby were 12plex antibiotic resistance at the most.Conclusions Salmonella germ-carrying rate of employees in food service industry had not changed in recent years,but it had gained resistant to commonly used drugs,so enhanced the detection work of enteropathogenic bacteria among employees,and carriers took medicine standardized could control foodborne disease.