胶体金法与13C、14C及胃镜HE染色检测幽门螺杆菌的对照研究

Contrast study on dot immunogold filtration assay,^(13)C,^(14)C,and HE in detecting Hp

目的探讨胶体金法检测幽门螺杆菌(Hp)感染的临床价值。方法将234例病人分为3组,其中13C尿素呼气(13C-UBT)试验组57例(A组),14C尿素呼气试验组79例(B组),HE染色试验组98例(C组),3组均同时与胶体金法作对照。结果 A组胶体金法与13C试验法阳性符合率为87.89%,B组胶体金法与14C试验阳性符合率为91.12%,C组胶体金法与HE染色试验法阳性符合率为89.29%。结论胶体金法检测幽门螺杆菌操作简单,是一较理想的非侵入性诊断Hp的方法。

Objective To explore the clinical value of Dot Immunogold Filtration Assay(DIGFA) in testing Hp infection.Method 234 patients were randomly selected and divided into 3 groups,in group A(57 cases),Hp was detected by 13C-UBT,in group B(79 cases),Hp was detected by 14C-UBT,in group C(98 cases),Hp was detected by HE.DIGFA were used for detecting Hp infection in all 3 groups.Results In group A,the positive coincidence rate of 13C-UBT and DIGFA was 87.9%.In group B,the positive coincidence rate of 14C-UBT and DIGFA was 90.9%.In group C,the positive coincidence rate of HE and DIGFA was 89.29%.Conclusions DIGFA is a kind of ideal and noninvasive method to detect Hp infection.