[Hg(SCN)_4]^(2-)配阴离子二级散射和倍频散射光谱法测定蛋白质

Determination of Proteins with [Hg(SCN)_4]^(2-) by Second-Order Scattering and Frequency Doubling Scattering Technique

在pH=1.0~4.5的稀硫酸介质中,[Hg(SCN)4]2-配阴离子能与人血清白蛋白(HSA)、α-糜蛋白酶(α-Chy)、牛血清白蛋白(BSA)、溶菌酶(Lyso)和γ-球蛋白(γ-G)等蛋白质反应形成复合物,此时将引起二级散射(SOS)和倍频散射(FDS)的显著增强.最大SOS峰和最大FDS峰分别位于668 nm和396 nm附近,散射增强(ΔI)在一定范围内与蛋白质的质量浓度成正比,两种方法对不同蛋白质的检出限(3σ)分别在17.30~43.53 ng/mL(SOS)和18.38~54.04 ng/mL(FDS)之间.据此发展了用SOS和FDS法灵敏、简便、快速测定血清中总蛋白的新方法.考察了共存物质的影响,结果表明该方法具有较好的选择性.

In H2SO4 solution of pH=1.0-4.5,[Hg（SCN）4]2-reacted with proteins such as human serum albumin（HSA）,α-chymotrypsin（α-Chy）,bovine serum albumin（BSA）,lysozyme（Lyso） and γ-globin（γ-G） to form complexes.As a result,the intensities of both second-order scattering（SOS） and frequency doubling scattering（FDS） were greatly enhanced.The maximum peak of SOS and FDS spectra was located at about 668 nm and 396 nm,respectively.The enhanced scattering intensity（ΔI） was proportional to the concentration of protein and the detection limits for the determination of different of proteins were in the range of 17.30-43.53 ng/mL for SOS and 18.38-54.04 ng/mL for FDS.Effects of coexisting substances showed that this method had a relatively good selectivity.A new method hereby using [Hg（SCN）4]2-as a probe for the determination of proteins in human serum was established.