肾癌患者外周血树突状细胞免疫状态变化及临床意义

Change of immunology function of dendritic cells of patients with renal carcinoma

目的探讨肾癌患者外周血中树突状细胞(DC)的免疫功能状态变化及其与肾癌发生的关系。方法 40例临床确诊的中晚期肾癌患者作为实验组,20例正常人作为对照组,实验组和对照组在相同条件下分别进行外周血DC的培养,并通过流式细胞仪检测CD1a、CD83表达水平。结果肾癌患者DC表面CD1a及CD83表达水平分别为55.6±1.3、71.2±1.2,明显低于正常对照组(65.0±1.1、81.3±1.6),P<0.05。结论肾癌患者DC存在免疫功能缺陷可能是肾癌逃避宿主免疫监视的重要原因之一。

Objective To investigate the change of immunology function of dendritic cells from renal carcinoma patients and its relationship with renal tumor.Methods To isolate and purify DC from peripheral blood of patients with renal tumor（n=40） and health controls（n=20）,detect the expression level of CD1a and CD83 costimulatory molecular on DC.Results The expression level of CD1a and CD83（55.6±1.3、71.2±1.2） on DC from patients was lower than that（65.0±1.1、81.3±1.6,P0.05） from contrasts.Conclusion The results indicate that the functional deficit of immune of the DC from renal carcinoma patient may be an important reason for tumor to escape from the immunology surveillance of tumor bearing host.