肝部分切除术促人结肠癌Caco-2细胞肝转移及相关机制

Partial hepatectomy promotes Caco-2 human colon cancer cells liver metastases and corresponding mechanism

目的研究肝部分切除术对人结肠癌Caco-2细胞肝转移的影响及其相关机制。方法应用人结肠癌Caco-2细胞系,构建结肠癌肝转移裸小鼠模型,同时行肝部分切除术(PH),假手术(Sham)设为对照,并对Caco-2细胞行siRNA-Snail干扰。观察肝转移灶面积,肿瘤组织Snail和基质金属蛋白酶2蛋白(MMP-2)表达。结果 PH组转移灶面积、Snail蛋白和MMP-2表达均显著大于Sham组和siRNA-Snail-PH组。结论 PH可促进结肠癌发生肝转移,该作用与促进Snail表达及上调Snail调控的MMP-2表达相关。

Objective To investigate the effects of partial hepatectomy to the liver metastases of Caco-2 human colon cancer cells and discover its mechanism.Methods In order to constructing the colorectal liver metastases nude mice model,the Caco-2 human colon cells were injected into nude mice through portal vein trunk to imitate the liver metastases pathway of colon cancer cells.Meanwhile,the partial hepatectomy（PH） was performed,and the Sham operation was set to control.Then the siRNA-Snail was imported into Caco-2 cells to interfere the expression of Snail mRNA;and area of the liver metastases,the expression of Snail and MMP-2 was detected.Results The metastases area,the expression of snail and MMP-2 of PH group was significantly higher than Sham group and the siRNA-Snail-PH group.Conclusion PH may promote the liver metastases of colorectal cancer,this closely correlates with up-regulating Snail expression and increasing its nucleus displacement and thus up-regulating MMP-2 expression.