胡桃楸提取液对肿瘤细胞增殖的抑制作用

The effects of the ethanol extract of Juglans mandshurica leaves on the proliferation of human tumor cells in vitro

目的考察胡桃楸提取液对肿瘤细胞Hela、K562的抑制作用和相关机制。方法用MTT方法分析胡桃楸提取液对Hela、K562细胞增殖的影响。采用端粒酶PCR ELISA试剂盒分析胡桃楸提取液对Hela、K562细胞端粒酶的影响。结果 Hela细胞24、48和72 h的LD50分别为406.18μg/mL、319.48μg/mL和112.84μg/mL。K562细胞24 h LD50为154.50μg/mL。HLF细胞LD50为918.69μg/mL。胡桃楸提取液可抑制Hela细胞和K562细胞的端粒酶活性,而对HLF细胞端粒酶活性影响不大。结论胡桃楸提取液对Hela细胞、K562细胞有抑制作用,在低浓度下对HLF细胞杀伤不大。对肿瘤细胞抑制作用可能与抑制端粒酶活性相关。

Objective To explore the effect and possible mechanism of the ethanol extract of Juglans mandshurica leaves on the proliferation of Hela cells and K562 cells.Method MTT was used to analyze the inhibition rate of Hela cell and K562 cell.PCR ELISA was used to analyze the effect of the ethanol extract of Juglans mandshurica leaves on telomerase activity of the Hela cells and K562 cells.Result The LD50 of Hela cells treated with ethanol extract of Juglans mandshurica leaves for 24 h,48 h and 72 h were 406.18 μg/mL,319.48 μg/mL and 112.84 μg/mL,respectively.The LD50 of K562 cells treated for 24 h was 154.50 μg/mL.The LD50 of HLF cells treated for 24 h was 918.69 μg/mL.The ethanol extract of Juglans mandshurica leaves inhibited the telomerase activity of Hela and K562 cells while had little effect on the telomerase activity of HLF cells.Conclusion The ethanol extract of Juglans mandshurica leaves can inhibit the growth of Hela and K562 cells while have little effect on HLF cells at low concentration;the inhibition may have correlation with the inhibition of telomerase activity.