纳米金对PCR扩增效率影响的机制探讨

Mechanism of the Effects of Gold Nanoparticles on Polymerase Chain Reaction Efficiency

近年来,纳米金粒子对聚合酶链式反应(polymerase chain reaction,PCR)的增效作用倍受关注,但是其具体的机制仍未明确提出。研究发现在PCR反应中,纳米粒子的增效作用是存在最佳浓度的,增加DNA聚合酶或者小牛血清蛋白(BSA)可以消除纳米金粒子导致的抑制。我们认为,纳米金粒子可能起到了类似于聚合酶β亚基的作用,提高了DNA聚合酶的延伸能力;而过量纳米金对PCR的抑制作用可能与纳米金结合单链DNA产生的位阻效应有关。

Gold nanoparticles （AuNPs） have been found to greatly enhance the polymerase chain reaction （PCR） efficiency in recent studies. However, the underlying mechanism is still unclear, though several hypotheses have been proposed in previous studies. It was found that AuNPs enhanced the PCR efficiency in a concentration-dependent manner. However, the addition of DNA polymerase or bovine serum albumin （BSA） could eliminate the inhibitory effects of ex- cess AuNPs. Based on these results we propose a possible mechanism that AuNPs might interact with polymerase in a manner analogous to 13-sliding clamp to modulate the activity of polymerase and improve PCR amplification ; while the excess AuNPs binding to ssDNA to produce a steric effect might inhibit amplification efficiency.